Coding

Part:BBa_K3201000:Design

Designed by: Anastasios Galanis   Group: iGEM19_Athens   (2019-08-31)


nCpf1 (RNA-guided DNA nickase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 196
    Illegal PstI site found at 1258
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 196
    Illegal PstI site found at 1258
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 380
    Illegal BglII site found at 1922
    Illegal BglII site found at 2231
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 196
    Illegal PstI site found at 1258
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 196
    Illegal PstI site found at 1258
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
    Illegal NgoMIV site found at 3466
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This version has an R1226A mutation, which inactivates the Nuc domain and transforms the endonuclease into a nickase. This version contains no STOP codon, because the protein was used in a fusion protein.


Source

From Acidaminococcus sp.

References