Coding
Part:BBa_K3201000:Design
Designed by: Anastasios Galanis Group: iGEM19_Athens (2019-08-31)
nCpf1 (RNA-guided DNA nickase)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 196
Illegal PstI site found at 1258
Illegal PstI site found at 3667
Illegal PstI site found at 3865 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 196
Illegal PstI site found at 1258
Illegal PstI site found at 3667
Illegal PstI site found at 3865 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 380
Illegal BglII site found at 1922
Illegal BglII site found at 2231 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 196
Illegal PstI site found at 1258
Illegal PstI site found at 3667
Illegal PstI site found at 3865 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 196
Illegal PstI site found at 1258
Illegal PstI site found at 3667
Illegal PstI site found at 3865
Illegal NgoMIV site found at 3466 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This version has an R1226A mutation, which inactivates the Nuc domain and transforms the endonuclease into a nickase. This version contains no STOP codon, because the protein was used in a fusion protein.
Source
From Acidaminococcus sp.